Repellent capacity of nanoparticles of ethanolic extracts of native bee propolis against Colaspis hypochlora

Abstract

Colaspis hypochlora is considered one of the main pests of banana fruit because it causes visible damage, such as black spots, negatively impacting the presentation and quality of these fruits destined for export. To counteract this problem, more environmentally friendly alternatives have been explored, therefore, the objective of this project is to determine the repellent capacity of ethanolic extracts of propolis from native Soconusco bees against C. hypochlora. In accordance with NOM-003-SAG/GAN-2017, propolis was obtained from the species Melipona beecheii and Apis mellifera. The total content of phenols and flavonoids was determined. The nanoparticles were prepared with the ionic gelation methodology, and the repellent activity of the ethanolic extracts of propolis, according to the sandbox with sensidisks procedure. The content of phenols and flavonoids for A. mellifera was 35.13±0.53 mg mL-1 and 5.92±0.19 mg mL-1, while for M. beecheii they were 17.69±0.05 mg mL-1 and 10.37±0.35 mg mL-1. The sudden activity of EEP nanoparticles from both species of bees was demonstrated, the highest repellent activity, in the case of M. beecheii nanoparticles at concentrations of 60 ng mL-1 for a time of 30 and 45 min, as To the A. mellifera nanoparticles, at a concentration of 40 ng mL-1 for 30 and 60 min, 86.67% repellency was obtained.