Nematicidal activity in vitro of three strains of edible mushrooms Pleurotus spp. against Haemonchus contortus (L3) and Nacobbus aberrans (J2)

Abstract

The objective of the present study was to evaluate the in vitro nematicidal activity of three strains of the mycelium of the edible mushroom Pleurotus spp. (P. ostreatus strain: 1123, P. eryngii strain: 1290 and P. djamor strain: 0127) against the nematodes Haemonchus contortus (L3) and Nacobbus aberrans (J2). The differents edible mushrooms (P. ostreatus strain: 1123, P. eryngii strain: 1290 and P. djamor strain: 0127) were cultivated in Petri dishes for 8 days. Subsequently, for the confrontation with the nematodes, series of 10 plates with mycelium of each edible mushroom and 10 plates without edible mushroom (control) were used. In the case of H. contortus, 2,500 infective larvae (L3) were added to each series of Petri dishes and this confrontation lasted for seven days. On the other hand, for the bioassay of the nematode N. aberrans to each series of Petri dishes with and without edible mushrooms, 300 larvae of N. aberrans (J2) were added and left in confrontation for 7 days. The results show a mortality percentage of H. contortus with 31.34% for the P. djamor edible mushroom, 87.43% with P. eryngii and 81.49% with P. ostreatus. Regarding the mortality of N. aberrans, the results show a mortality rate of 76.77% for P. djamor, 68.05% with P. ostreatus and 13.19% with P. eryngii. Pleurotus spp. fungi species used in this study have nematicidal activity. P. ostreatus showed significant anthelmintic activity against H. contortus (L3) and N. aberrans (J2).