In vitro culture of 22 wild genotypes of chili from the Región Selva de Chiapas, Mexico

Abstract

Plant genetic resources are the basis of the food security for the world population. Mexico is one of the centers of origin and diversity of several phytogenetic species, including various types of chili. The majority are wild species distributed in most of Mexico and of wide cultural and economic interest. The objective of this study was to determine the optimal concentration of indole-3-acetic acid (IAA) and kinetin (KIN) for the multiplication of seedlings shoots and roots of 22 genotypes of wild chili regenerated from seeds germinated in vitro. Under a completely randomized design, the seeds were germinated in medium of Murashige and Skoog (MS), later they were transplanted into flasks containing 30 cm3 of MS medium supplemented with 0.0/0.0, 1.0/0.3, 0.3/0.5, 0.5/1.0 mg L-1 of KIN/ IAA. A maximum average of 9.68 shoots and 10.90 of roots were obtained with 0.5 / 1.0 mg L-1 of KIN/ IAA. The addiction of growth regulators promoted the induction of shoots and roots in the 22 wild genotypes of chili.